Molecular and Genetic Medicine

A New Era in Functional Genomics Using CRISPR/Cas9 Knockout Screening

Abstract

Wenzhi Cai1, Weijing Li, Dan Yang, Huafeng Xie and Jian Huang

In this commentary, we discussed the new exciting progress in CRISPR based screening technology field and highlight recent developments in the area of CRISPR-based functional genomics. High-throughput functional genomics using CRISPR-Cas9 revolutionized our ability to decipher cellular function in health and disease. Despite its limitations, the simplicity and effectiveness of CRISPR/Cas9 based screening, makes an enormous impact on genomic screening and thus scientific discovery.