Microfluidic devices are essential tools in various scientific and industrial applications, including chemical analysis, medical diagnostics, and biosensing. These devices, which manipulate small volumes of fluids at the micron scale, offer significant advantages over traditional laboratory equipment, such as faster reaction times, lower reagent costs, and the ability to conduct experiments with minimal sample sizes. One of the critical factors in enhancing the performance of microfluidic devices is the optimization of the optical path length. The optical path length refers to the distance that light travels through a medium, and its length can significantly influence the accuracy and sensitivity of optical measurements. In many applications, such as spectrophotometry or fluorescence analysis, a longer optical path length increases the interaction time between the light and the sample, improving the sensitivity and detection limits of the device. A promising technique for increasing the optical path length in microfluidic devices is the use of a multi-pass cell.
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