Plants are a possible source of antimicrobial agents in different countries. Medicinal plants are usually in the past, used for the treatment of human infections. The present study was tried to find the truth about Gymnema sylvestre and Nyctanthes arbor-tristis for their possible activity against disease-causing bacteria. Gymnema sylvestre and Nyctanthes arbor-tristis extracts were prepared in hot water, some organic solvent like methanol and chloroform. The activity of plant extracts and selected germ-killing drugs were against three bacterial including staphylococcus aureus, bacillus subtilis, and Pseudomonas aeruginosa using the clear agar well diffusion method. Among the two medicinal plants, organic solvent extracts displayed possible activity against methanol extract of these 2 medicinal plants showed the highest activity against staphylococcus aureus, bacillus subtilis, and Pseudomonas aeruginosa which is compared with a clear zone of inhibition using azithromycin and DMSO. These 2 extracts show germ-killing activity. Further studies are needed to explore the novel design drugs in the future.
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