Uzair Ahmad
Background and Objective: Contamination with aflatoxins in raw milk is a burning issue in the World regarding human health. Aflatoxins are secondary metabolites of toxigenic fungi, and are potential mutagens, carcinogens, teratogens, genotoxins and other health hazards to human. These toxins are produced by fusarium, penicillium and aspergillus Spp. Aflatoxin M1, hydroxylated forms of Aflatoxin B1 (AFB1), predominantly present in milk.
Materials and methods: ELISA technique was us to check the quantity of aflatoxin M1 in milk samples. Aflatoxin M1 test kit was used for the detection of AFM1 in milk by carrying out the procedure of Competitive Enzyme Linked Immunosorbent Assay (ELIZA). Competitive Enzyme Linked Immunosorbent Assay (ELIZA) is a cost effective, quick and reliable technique for the quantitative analysis of AFM1. German Federal board of Health (Kaniou-Grigoriadou, Eleftheriadou, Mouratidou, & Katikou, 2005) also included this process in their official collection of test procedure.
Results and discussion: The results showed that every sample was contaminated with AFM1. The results from the test shows that every sample was contaminated with AFM1. According to the findings of different farms all samples were found positive with Aflatoxin M1 but only 15% were found higher value than European Commission while all 100% samples were below value according to the Pakistan Standard and Quality Control Authority acceptable limits Maximum concentration was shown in Seth Shahmeer Dairy Farm and Zumurd Dairy Farm 0.585 μg/l and minimum in Al Hadi Dairy Farm with value 0.252 μg/l.
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