Molecular Biomarkers & Diagnosis

Blocking Agent of Heme Oxygenase-1 Zinc Protoporphyria Induces Autophagy and Accelerates Oxidative Damages during Lipopolysaccharide-Induced Lung Injury in Rat

Abstract

tY Gao, Y Zhang and J Zhang

To investigate the effect of blocking agent of heme oxygenase-1 zinc protoporphyrin (Znpp) on lipopolysaccharide (LPS)-induced autophagy in acute lung dysfunction, the rats were divided into control (C), LPS (L), LPS +Hemin (Hemin) and LPS+ZnPP (ZnPP) groups. Treatment with ZnPP induced autophagy and accelerated oxidative damages during lipopolysaccharide treatment in rat lung, LPS+ZnPP increased pathological alterations in lung tissues, the number of ballooned pulmonarycytes, serum tumor necrosis factor (TNF)-α and interleukin (IL)-6 levels, and myeloperoxidase (MPO) and malondialdehyde (MDA) levels in lung tissues (P < 0.05) but attenuated by LPS +Hemin treatment. Thus, ZnPP may aggravate LPS-induced acute lung dysfunction in rats, possibly by increasing inflammation and accelerating oxidative damages. LPS+Hemin group prolonged the median survival time and reduced lung dysfunction. Moreover, HO-1 may significantly contribute to lung protection.