Journal of Phylogenetics & Evolutionary Biology

Colchicine Induced Polyploidy in Chrysanthemum carinatum L.

Abstract

Kalyan Singh Kushwah, Rakesh Chandra Verma, Sapan Patel1 and NK Jain

Induction of colchitetraploidy was attempted in local Chrysanthemum carinatum (2n=18). The method used for colchicine treatment was “cotton swab method”. The details about the successful induction of polyploidy, morphological features and cytological (meiosis) are described and compared with control characters. The phenotypic characters observed such as slightly slower growth, stronger stem, thicker and larger leaves, lager flowers and seeds in the colchitetraploid plant. Cytological characters also such as increase cell size, chiasma frequency, DNA contents and double chromosome number in colchitetraploid plant compared with control. The growth pattern, morphology, pollen stain-ability and seed set were recorded; however, main emphasis was given on chromosomes behavior of control and isolated colchitetraploidy.