Journal of Bioengineering & Biomedical Science

Contribution of EDTA and Shaping Technique in the Quality of Teeth Cleaning Infected: In Vitro Study

Abstract

Najah NF, Sid R and Ghodbane N

Study objective: The aim of our study was to compare the effectiveness of EDTA cleaning gel to 17% when combined with sodium hypochlorite (NaClO) to 2.5% during canal preparation, with respect to the use of NaClO alone. By using two preparation techniques, the manual technique using manual files steel and continuous rotation with the Hero-shapers system.
Materials and methods: The study was conducted in vitro on infected teeth, freshly extracted single rooted. All teeth were carefully cleaned and sectioned at the JCD.
On each tooth, a bacteriological sample was taken before and after treatment canal with sterile paper points and analysed according to microbiological standards.
The study was conducted on a total of 84 teeth randomly divided into 4 groups of 21 teeth each as follows:
The first group was prepared manually with steel instruments and received sodium hypochlorite at 2.5% as irrigating; the 2nd group was manually prepared with the instruments steel and received sodium hypochlorite at 2.5% associated with EDTA gel at 17% as irrigating; the 3rd group was prepared with rotary instruments Hero Shaper and received sodium hypochlorite at 2.5% as irrigating; the 4th group was prepared with rotary instruments Hero Shaper and received sodium hypochlorite at 2.5% associated with EDTA at 17% as irrigating. The results of bacteriological analysis were processed using Epi Info 3.3.2 software, retained significance index p <5%.
Results: 23% of germ eradication for the teeth of the first group, 56% for the 2nd group, 47% for the 3rd group and 68% for the 4th group.
Conclusion: The use of a chelating agent undeniably improves the quality of cleaning of the root canals, compared with the use of only sodium hypochlorite.