In prokaryotes, the clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages. This system inspired the development of the CRISPR/Cas9 genome editing system, which is a powerful genome engineering tool. The CRISPR/Cas9 technique has been used to investigate the functions of cancer-related genes, establish tumor-bearing animal models and probe drug targets, greatly increasing our understanding of cancer genomics. In this paper, we examine the current state of CRISPR/Cas9 gene editing technology in oncological research. We begin by explaining the fundamental principles of CRISPR/Cas9 gene editing before introducing several new CRISPR-based gene editing modes. The rapid progress of CRISPR screening in revealing tumorigenesis, metastasis and drug resistance mechanisms is then described.
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Journal of Genetics and DNA Research received 3 citations as per Google Scholar report
