Journal of AIDS & Clinical Research

Detection of Cytomegalovirus Glycoprotein B Genotypes among Early and Late Stages of HIV Infected Sudanese Patients

Abstract

Mahjoob O Mahjoob*, Shamson K Kafi, Abu Elgasim A Alsidig, Haitham E Elawad, Mustafa H Mustafa, Alteleb G Elnaim and Mohammed EH Ournasseir

The present research was conducted at the voluntary counseling and testing (VCT) center Khartoum, Sudan during 2015-2016. This is a cross-sectional study. A sum of 194 individuals were recruited to take part in this research (of them 96 were HIV patients in the first stage and 98 in the late stage of infection). Blood specimens were collected from each subject. The specimens were screened for cytomegalovirus CMV-antibodies (IgM and IgG) using ELISA (Acon Laboratories, Inc.), CMV-DNA was determined by Polymerase chain reaction PCR and CMV-gp (glycoprotein) B genotyping was performed by Restriction fragment length polymorphism (RFLP). Results of this investigation showed a significant prevalence of CMV infection. The results of HIV cases co-infected with CMV showed up that the disease is higher among females than males and among cases in late-stage more than those in the first stage. The age cohort 9-31 years, subjects on treatment and HIV cases with clinical manifestations displayed significant seroprevalence of CMV-IgM. CMV-gpB1 genotype was more frequent among sufferers in the late stage of disease, subjects with clinical manifestations and females. CMV-gpB2 and 3 genotypes were more frequent in males. We concluded that CMV and its related gpB genotypes may influence the diagnosis and outcome of AIDS cases.