Veterinary Science & Technology

Development of a Method to Detect Viral RNA Sequences From Cultured Cells by Combining Size Fraction and a Rapid Determination System for Viral RNA Sequences (RDV)

Abstract

Shumpei Watanabe, Tetsuya Mizutani, Kouji Sakai, Itoe Iizuka, Tomoyuki Shiota, Yusuke Sayama, Shumpei Tsuda, Kentaro Kato, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa and Hiroomi Akashi

By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as “RDV-size fraction (RDV-SF)”. Using RDV- SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.