Nandini N. M
Liquid based cytology (LBC) is a procedure wherein cells are spread in a monolayer with a clean background. It is usually automated, semi-automated and manual
method. Manual liquid based cytology (MLBC) is an indigenous, cost effective method of cytology which we have used in our setup of low middle income group
country (LMIC). Also it helps us to use the residual sample for study of ancillary techniques like cell block (CB), immunocytochemistry, ICC and IHC on MLB and
CB respectively. Molecular tests like HPV can also be performed.
Aim of the study: to study the utility of MLBC in cervical, breast and thyroid lesions. To use ancillary techniques like CB and Molecular techniques like PCR to
improve diagnosis by use of ICC or IHC wherever possible. MLBC was done by using an in house method by use of polymer solution. The systems studied were
pap smear screening for cervical lesions, breast, and thyroid. The study was conducted over a period of 5 years till 2020, covering each aspect of MLBC in step
wise manner. ICC was conducted on both cervix (p16marker) and breast (ER).IHC was done on CB for cervix (p16, ki67) and breast (ER, PR, HER2).
The results for all the three systems showed good results giving a better diagnostic accuracy of non-neoplastic and neoplastic conditions. MLBC is a complimentary
test for routine screening procedure like pap smear screening and FNAC of breast and thyroid. It helps to give a better diagnosis as it helps to remove obscuring
factors like blood and inflammatory cells.
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