The increasing demand for reliable and rapid detection methods in food safety has led to the exploration of innovative technologies. Nanoporebased sequencing platforms represent one such promising advancement, offering the capability for real-time, high-throughput, and portable analysis. These platforms leverage nanopore technology, where single molecules of DNA or RNA pass through a nanopore, generating an electrical signal that is subsequently translated into a nucleotide sequence. This study investigates the quantification potential of such a platform for food safety applications, focusing on the use of external standards like lambda DNA and lambda-spiked beef samples. Lambda DNA, derived from the bacteriophage lambda, serves as a model system in molecular biology due to its well-characterized genome. It provides a reliable external standard for evaluating the performance and quantification capacity of nanopore sequencing systems. The uniformity and stability of lambda DNA make it an ideal candidate for calibration and performance assessments. In this context, lambda DNA was utilized to assess the precision, reproducibility, and sensitivity of a nanopore sequencing platform, laying the foundation for its application in food safety
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