GET THE APP

..

Journal of Hypertension: Open Access

ISSN: 2167-1095

Open Access

Expression and Importance of Interleukin-17 and Transforming Growth Factor-�Ž�² in the Spleens of Hypersplenic Rats

Abstract

Yunfu Lv, Qingqing Li, Jie Deng and Shuyong Yu

Objective: We analyzed the expression of interleukin-(IL)-17 and transforming growth factor (TGF)-β in the spleens of rats suffering from liver cirrhosis and hypersplenism, and investigated the cause of peripheral cytopenias.
Methods: Fifty-five male Sprague–Dawley rats were divided randomly into two groups. The control group (n=15) was gavaged with physiologic (0.9%) saline. The experimental group (n=40) was gavaged with 40% CCL4/peanut oil solution at 0.3 mL/100 g twice a week for 8 weeks, and a 15% alcohol solution was given as drinking water, to establish a model of liver cirrhosis and hypersplenism. After confirmation of liver cirrhosis and hypersplenism, spleen tissue was collected to determine expression of IL-17 and TGF-β by Immuno Histo Chemical (IHC), western blotting, and real-time fluorescence-based quantitative polymerase chain reaction (PCR).
Results: IHC staining revealed positive expression of IL-17 and TGF-β in the experimental group to be 36.67% and 73.33% respectively, which was significantly higher than that in the control group (6.67% and 13.33%, respectively; χ2=4.60, P<0.05; χ2=14.46, P<0.01). Western blotting revealed the relative expression of IL-17 and TGF-β in the experimental group (1.09 ± 0.39, 1.51 ± 0.22) to be significantly higher than that in the control group (0.53 ± 0.25, 0.63 ± 0.17) (t= -3.227, P<0.01; t= -9.264, P<0.01). Real-time fluorescence-based qPCR showed relative expression of IL-17 and TGF-β mRNA in the experimental group to be 2.81 ± 0.70 and 2.91 ± 0.63, respectively, which was significantly higher than that in the control group (1.06 ± 0.21 and 0.99 ± 0.052, respectively) (t= -5.96, P<0.01; t= -7.35, P<0.01). Pearson correlation analyses showed the relative mRNA expression of IL-17 to be positively correlated with that of TGF-β (R=0.520, P<0.01).
Conclusion: Expression of IL-17 and TGF-β was increased significantly and positively correlated in the spleens of rats suffering from liver cirrhosis and hypersplenism, which may have caused peripheral cytopenias.

PDF

Share this article

Google Scholar citation report
Citations: 614

Journal of Hypertension: Open Access received 614 citations as per Google Scholar report

Journal of Hypertension: Open Access peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward