Journal of Microbial Pathogenesis

Green Synthesis of Cobalt and Copper Nanoparticles from Extract of Rumex Hastatus and Their Biological Evaluation

Abstract

Taj Ur Rahman*, Babar Hussain, Abdul Hameed, wajiha Liaqat and Shehriyar Khan

The cobalt and copper nanoparticles are gaining wider attention due to their applications in medicines, chemistry, biotechnology and agriculture. In the present study, cobalt and copper nanoparticles were synthesized by green technology using leaf extract of Rumex hastatus and characterized by Ultraviolet-visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) technologies. The synthesis of nanoparticles were observed by colour change of reaction mixture and UVVisible spectrum showed maximum absorption peaks at 620 nm and 570 nm for copper and cobalt nanoparticles respectively. The FT-IR spectrum of Rumex hastatus leaf extract showed prominent peaks at 3240-2400 cm-1 (O-H stretch), 1710 cm-1 (C=O stretch), 1654cm-1 (C=C stretch) and 1618cm-1 (C=C stretch). However, these peaks were absent in the spectrum of cobalt and copper nanoparticles, meaning that these functional groups are involved in the reduction of cobalt and copper ions into their metallic nanoparticles. The SEM result showed irregular, spherical shape crystallites with rough surface. The average size for cobalt and copper nanoparticles were found in 52 nm and 78 nm respectively. The cobalt strongly inhibits the growth of P.aereginosa while copper nanoparticles strongly inhibit the growth of E.coli. The experimental data reveals that cobalt nanoparticles have more α-amylase inhibition activity than copper. The anti-glycation activity of cobalt nanoparticles is more than copper nanoparticles while copper nanoparticles have more anti-oxidizing potential than cobalt nanoparticles.