Journal of Antimicrobial Agents

Green Synthesis of Silver Nanoparticles Using Piper nigrum Concoction and its Anticancer Activity against MCF-7 and Hep-2 Cell Lines

Abstract

Manikandan E,Krishnan V, Bupesh G, Thanigai Arul K, Magesh S, Kalyanaraman R and Maaza M,

In the present report, silver nanoparticles were synthesized using Piper nigrum extract for in vitro cytotoxicity efficacy against MCF-7 and Hep-2 cells. The silver nanoparticles (AgNPs) were formed within 20 minutes and preliminarily confirmed by UV-Visible spectroscopy. Further, it was characterized by FT-IR and HR-TEM. The UVVisible analysis showed the strong broad peak located at 441 nm observed for Ag nanoparticles. TEM images of biosynthesized AgNPs pre-dominantly spherical shape with particle size in the range 20 nm. MTT assays were carried out using various concentrations of silver nanoparticles and Piper nigrum extract ranging from 10 to 100μg/ml. At various concentrations, biosynthesized silver nanoparticles showed a significant cytotoxic effect against both MCF-7 and Hep-2 cells compared to Piper nigrum extract. Therefore, the results reveal excellent applications of green synthesis of silver nanoparticles using Piper nigrum