Veterinary Science & Technology

Identification and Antimicrobial Susceptibility Profile of Salmonella Isolated from Selected Dairy Farms, Abattoir and Humans at Asella Town, Ethiopia

Abstract

Takele Beyene, Habtie Yibeltie, Bulako Chebo, Fufa Abunna, Ashenafi Feyisa Beyi, Bedaso Mammo, Dinka Ayana and Reta Duguma

Salmonella is one of the most common causes of food-borne diarrheal disease in human as well as animals. It is leading causes of acute gastroenteritis when ingested in contaminated foods, including meat and dairy products. Moreover, the emergence of multiple-resistant (MDR) isolates is increasing in human and veterinary medicines. Therefore, this cross-sectional study aims at isolation, identification and antibiogram of Salmonella from selected dairy cattle farms, abattoir and in contact humans in both dairy farms and abattoir of Asella, Ethiopia. We collected 185 samples from abattoir (n=94) and dairy farms (n=91), which were isolated and identified according to ISO-6579, 2002. The overall proportion of Salmonella was 6.5% (12/185) (dairy farms n=4, 4.4% and abattoir n=8, 8.5%). Antibiogram of isolated Salmonella was also evaluated against ten commonly used antibiotics in both humans and veterinary medicines to treat salmonellosis by using the Kibry Bauer disk diffusion method. All isolates (100%, n=12) were susceptible to ciprofloxacin and gentamycin followed by 91.7%, 75%, 66.7%, 58.3 and 50% of the isolates were susceptible to sulfamethoxazole-trimethoprim, chloramphenicol, kanamycin, nalidixic acid and streptomycin, respectively. However, cefoxitin showed the highest resistance (66.7%) followed by ampicillin and amoxicillin (58.3% each). Moreover, 50% of the isolates were resistant to two or more of the tested antimicrobial agents. The highest MDR was seen on polled hand swabs from abattoir, resistance to eight antimicrobials (80%, n=8/10) with the combination of cefoxitin, ampicillin, amoxicillin and streptomycin being more frequent. High proportion of Salmonella was isolated from abattoir sample than dairy farms. These isolate developed MDR to commonly prescribed antimicrobial agents in the study area. Hence, strict hygienic management in the farm and abattoir as well as rational use of antimicrobials should be practised to circumvent the further development of antimicrobial resistance.