Journal of Tissue Science and Engineering

In Vitro Production of Sheep Embryos in CR1aa Medium Supplemented with L-Ascorbic Acid

Abstract

Dulam Sreenivas, DSVGK Kaladhar, Nagendra Sastry Yarla, VM Thomas, A PalniSamy, Varahala Rao Vadlapudi and R Preethi

In vitro embryo-production procedures developed for sheep have tremendously been improved from decades,
but many factors influencing their efficiency still need to be investigated. The overall of this study showed the
production of the sheep embryos till blastocyst stage from the ovaries of the slaughtered ewes for IVM, IVF of the
oocytes and then IVC in the complex culture media like TCM-199, TCM-199-, TCM-199+ and CR1aa. The morula
yield was significantly higher in the wheat peptone and BSA supplemented group. The nuclear maturation rates of
ovine oocytes matured in FBS (69.15 ± 1.07) group was more compared to BSA (56.47 ± 0.73) and wheat peptone
(35.26 ± 0.79). The nuclear maturation of oocytes was significantly higher in the FBS group when compared to BSA
and wheat peptone supplemented groups. The lowest maturation rate was observed in wheat peptone supplemented
group. The development rates in 2, 4, 8, 16 and morula stages of ovine embryos produced from BSA group were high
(74.22 ± 2.56, 60.93 ± 2.55, 42.97 ± 2.20 and 24.21 ± 1.28) compared to FBS group (73.58 ± 2.92, 58.77 ± 2.01,
41.22 ± 1.68 and 20.17 ± 0.76) and wheat peptone (72.89 ± 1.09, 57.01 ± 0.76, 42.05 ± 0.36 and 24.29 ± 0.55). The
cleavage, morula and blastocyst percent in CR1aa was found significantly high compared with TCM- 199, TCM-199-
and TCM-199+. The cleavage, morula and blastocyst percent in L-Ascorbic acid at 100 μM with CR1aa is found more
significant compared with other compositions used in the experimentation.