Hydrology: Current Research

Inactivation and Structural Changes of E. Cloacae and B. Subtilis Endospores during IR Laser Water Treatment

Abstract

S. Ede, L. Hafner, R. Frost and G. Will

The efficiency of CO₂ infrared (IR) laser irradiation to disinfect water containing E. cloacae and B. subtilis endospores was determined and compared to ultraviolet (UV) treatment. Cell viability decreased with IR laser treatment for E. cloacae; however, increased viable cell concentrations were observed in B. subtilis endospores. Fourier Transform Infrared (FTIR) spectroscopy and the chemometric method of Principal Component Analysis (PCA) were used to examine cell changes. Increased carbohydrate content was observed in E. cloacae after IR laser treatment.