Zafar Mehmood, Arunesh K Dixit, Ajay Singh, S Farooq and Suman Lata Chhimwal
The results of present study support the view that Hemidesmus indicus and its isolated bioactive compound (2H4MB) showing potential antibacterial and antifungal activities that could be utilized to control infectious diseases caused by the test pathogens and Candida albicans in the human system. Analysis of essential oil from root of the plant obtained by using Clevenger apparatus by hydro distillation was undertaken through GC-MS. 2-Hydroxy- 4-methoxybenzaldehyde (2H4MB) was the major chemical entity (99.41%) amongst 10 identified compounds. The in vitro antibacterial activity of 3 different extracts (Hexane, Methanol and Aqueous) and Bioactive compound 2-Hydroxy-4-methoxybenzaldehyde (2H4MB) as well as MIC values of isolated compound (2H4MB) were performed. The most active extract was found to be the hexane extract showing the maximum zone of inhibition of 22 mm against Staphylococcus aureus. The isolated bioactive compound (2H4MB) showed the highest diameter of zone of inhibition of 23 mm against Staphylococcus aureus. The Minimum Inhibitory Concentration values of the Bioactive compound 2H4MB on test pathogens varies from 80 μg/ml to 250 μg/ml, Minimum Bactericidal Concentration values varies from 150 μg/ml to 250 μg/ml. The MFC values and MIC values of 2H-4-MB on Candida albicans were 200 μg/ml and 150 μg/ml respectively. The active extract was also tested for cytotoxicity using freshly obtained sheep erythrocytes and it was revealed zero cytotoxicity.
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