Biosensors & Bioelectronics

Nanoparticles Effects on Performance of Horseradish Peroxidase Biosensor

Abstract

Saghafi E and Farahbakhsh A

The immobilization of horseradish peroxidase (HRP) enzyme causes protections on catalytic activities in determination of H₂O₂. The rate of electron transfer from redox enzyme onto the electrode surface is an important topic in bioelectrochemistry that could improve more the function of HRP biosensor by means of increasing electrode conductivity. Referring to this basis, effect of various nanoparticles are evaluated and compared with each other on the operation promotion in this review and Glassy Carbon Electrode (GCE) is utilized as a main foundation on immobilization of nanoparticles and HRP enzyme. Electrodes have been compared by the rate of current transfer in the optimum voltage data in the presence of Carbon-coated Iron Nanoparticles (CIN), Quantum Dots (QDs), Au and Laponite/Chit nanoparticles. Consequently in the same scan rate, HRP/Au/GCE (28 μA) has achieved more current which is about 94% comparing to the base electrode HRP/GCE (1.8 μA).