Biosensors & Bioelectronics

Needle-Type Glucose Sensor Based on Functionalized Graphene

Abstract

K. ul Hasan, M. H Asif, O Nur and M Willander

We demonstrate a novel, highly efficient glucose sensor based on functionalized graphene. Glucose oxidase (GOD) immobilization has been apprehendedbythe direct interaction between carboxyl acid groups of the reduced graphene oxide (RGO) and amines of GOD together with the electrostatic interactions existing between the positively charged polymeric ionic liquid (PIL) and GOD. This combined system can provide a favorable microenvironment for the GOD to retain its good bioactivity. The enzyme-coated graphene biosensor exhibited glucose-dependent electrochemical measurements against an Ag/AgCl reference electrode. The resulting sensor show broad range detection, up to 100 mM glucose concentration, with a sensitivity of 5.59 μA/ decade. It was found that glucose biosensor based on functionalized graphene can be seen as an effective candidate for the detection of sugar concentration in clinical diagnoses.