Journal of Oncology Translational Research

Novel, Safe, Cheap, and, easily to get ʻ Vinegar as a decalcifying agent ʼ for bone tissue Histopathological demonstration

Abstract

Nesreen M. Safwat

Bone is the strongest part in the human and animals, as well examination and reporting any histopathological changes of the different bone tissues is quite difficult than any other tissues, and to obtain satisfactory paraffin or celloidin of bone, the inorganic calcium must be removed (Jimson et al., 2012) through the decalcification process. There are numbers of decalcifying agents used in both veterinary pathology and histology research laboratories (Gayle Callis, 1998). So choosing the appropriate one depend upon some factors such as; time for decalcification with ribboning of sections, good nuclear staining and minimal edema (Bancroft and Gamble, 2016). I used a novel, household, safe solution which is the commercial table vinegar; the method was: an egg as a model (outer egg shell), sunken into commercial table vinegar solution Hienz ®, the decalcification resulted after only one day. After that a rabbit mandible was used to monitor the effect of vinegar on bone sample and inspected daily, the results come matching the outer egg shell but it took 28 days. So, I conclude that; may use the commercial table vinegar which contain 4-8 % acetic acid as a novel, safe, cheap, available decalcifying agent in both histology and pathology laboratories.