Medicinal Chemistry

Outcome of Capparis Spinosa Fruit Extracts Treatment on Liver, Kidney, Pancreas and Stomach Tissues in Normal and Diabetic Rats

Abstract

Mohammad Mohsen Taghavi, Mahmood Nazari, Rasoul Rahmani, Ahmadreza Sayadi, Mohammad Reza Hajizadeh, Mohammad Reza Mirzaei, Hassan Ziaaddini, Seyed Mostafa Hosseini- Zijoud and Mehdi Mahmoodi

Aim and objectives: Herbal drugs such as, Capparis Spinosa (CS) has been used to treat several diseases from heart and cardiovascular disorders to diabetes in traditional Iranian medicine. The present study was aimed to determine beneficiary effects of CS fruit extract on a number of tissues in healthy and type 1 diabetic rat.
Materials and methods: In this experimental study, sixty male wistar rats were randomly divided into 6 groups (n=10) and subjected to gavage tube therapy for one months by following protocol: group I and IV: diabetic control and non-diabetic control rats which only received distilled water; groups II and III: non diabetic treatment rats which received 200 mg/kg and 800 mg/kg CS extract per day respectively; groups V and VI: diabetic treatment rats which received 200 mg/kg and 800 mg/kg CS extract per day respectively. Diabetes was induced by intra-peritoneal injection of 50 mg/kg of streptozotocin (STZ). After 4 weeks, their blood were collected and the serum levels of creatinine, bilirubin, urea, uric acid, AST, ALT and ALP were measured. The liver, kidney, pancreas and stomach tissues were immediately excised, and after slide preparation processes, the histological changes were studied.
Results: Our results illustrated that the gastric tissue in the diabetic group showed a small degree of changes and was not affected significantly by administration of the CS fruit extract. Liver, pancreas and kidney of diabetic rats exhibited considerable changes, like cellular necrosis. These changes in diabetic treatment groups were at lowest amounts. The decrease levels of creatinine, liver enzymes, and other factors were supporting these changes.
Conclusions: This study demonstrates that the CS fruit extract could be aid prevention of damage to the tissues due to the decreased levels of harmful oxidants in the body in diabetes. Moreover, according to our previous results altogether we showed beneficial impact of the plant on the treatment of diabetes.