Journal of Bioanalysis & Biomedicine

Production of Extracellular Pectinase by Bacillus Cereus Isolated From Market Solid Waste

Abstract

Elangovan Namasivayam, John Ravindar. D, Mariappan K, Akil jiji, Mukash Kumar and Richard L Jayaraj

Pectin is a major component of primary cell wall of all land plants and encompasses a range of galacturonic acid rich polysaccharides. Pectinase or pectinolytic enzyme, hydrolyzes pectic substance, they have a share of 25% in the global sales of food enzymes. Production of this enzyme is affected by the nature of solid substrate, level of moisture content, presence or absence of carbon, nitrogen, minerals and vitamin supplements. Maximum enzyme production (44U/ml) is achieved when temperature is around 37^°C, pH.8.5, pectin as a carbon source, yeast extract as a nitrogen source and incubation time is 36 hours. In addition to this, wheat bran acts as a better agro substrate and Magnesium chloride as supplement for better production of pectinase. The sequence analysis of 16S rDNA of the isolated organism was much similar when compared with gyrase B gene (gyrB) of Bacillus cereus. Pectinolytic enzyme is of significant importance in the application of apple juice industry in the current biotechnological era.