Journal of Tissue Science and Engineering

Production of Sterilized Medium Chain Length Polyhydroxyalkanoates (Smcl- Pha) as a Biofilm to Tissue Engineering Application

Abstract

Anteneh Getachew, Admas Berhanu and Andualem Birhane

The current increase in the utilization of polyhydroxyalkanoates (PHAs) in various industrial and biomedical applications is due to their biodegradability, compatibility, resorbability and piezoelectricity. In the present study, we developed a modified medium chain length PHAs (Mmcl-PHA) by sterilizing surfaces of PHAs extracted from bacterial isolate. FTIR analysis of the neat polymer confirmed the presence of functional groups corresponding to alkyl halide, alkyne, hydroxyl group, and alkane groups. Sterilization of PHA using ethylene oxide as a medium was resulted modification of minor band differences in the absorption spectrum of homopolymer of PHB (scl-PHA) in to the co polymer of P (HB-co-HV) medium chain (mcl-PHA). The variation on to carbonyl (C=O) ester groups modified without significant changes to physico-chemical properties of the polymer were noticed. Sterilization of PHA using ethylene oxide has been evident surface modification properties suited to tissue engineering application of scaffold fabrication.