Biosensors & Bioelectronics

Reversible Inhibitor Biosensor Systems in Dynamic Mode

Abstract

Vania Rangelova

The biosensor amperometric transducers can work in the case of three basic types of reversible inhibitor enzyme systems – with competitive inhibition, with non-competitive inhibition and mixed inhibition. Typically they work in static mode. Now they are investigated in dynamic mode. The kinetic in those type biosensors is generally discussed in terms of a simple extension to the Michaelis-Menten reaction scheme. The investigated biosensors are amperometric product sensitive. The parameters for simulations are chosen from some real experiments with biosensors. The models are described in non-stationary diffusion conditions. Solving system of non-linear partial differential equations is received in three dimensional size and the concentration profiles in active membrane of substrate S(x,t), inhibitor I(x,t) and product P(x,t) are received. The systems of non-linear differential partial equations have been solved numerically in MATLAB medium. The influence of starting concentration of substrate, inhibitor and kinetic parameters - reaction rate and reaction constants of biosensors over output current has been investigated.