The advent of messenger RNA (mRNA) therapeutics has revolutionized the field of medicine, particularly in the treatment of genetic disorders and infectious diseases. However, the complexity of mRNA molecules necessitates precise and reliable analytical techniques to ensure their efficacy and safety. Traditional chromatographic methods for mRNA analysis often fall short in terms of resolution and throughput. This article introduces a novel approach to mRNA analysis using ultra-wide pore Size Exclusion Chromatography (SEC) columns, which promise to enhance the precision of mRNA characterization. By expanding the pore size range of SEC columns, this technique improves the separation and quantification of mRNA molecules based on their size and conformation. This article discusses the principles behind ultra-wide pore SEC, compares it with conventional methods, and highlights its advantages in terms of resolution, reproducibility, and sensitivity. The implementation of these advanced columns can significantly advance mRNA therapeutics development and quality control.
HTML PDFShare this article
Pharmaceutical Regulatory Affairs: Open Access received 533 citations as per Google Scholar report
