The histochemical analysis of the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days (test group= TG) on hosts (New Zealand White rabbits) previously immunized with glandular extract of salivary glands obtained from females fed for 6 days (SGE6) was carried out in order to detect alterations in the secretion of glycoproteins, lipoproteins, acid phosphatase and calcium. It was here verified that the composition of the secretion produced by the glands of females from TG (2-6 days) was different from the one observed in those from control group (CG), with a gradual reduction in the synthesis of the components as feeding progressed, except for the proteins and calcium, which remained unaltered within the interval of 4 to 6 days and 2 to 4 days respectively. In the glands of females fed for 2 days (TG) there was increase in calcium staining, as well as a reduction in lipids and acid phosphatase, and staining for proteins and polysaccharides remained unaltered. In the females fed for 4 days (TG) there was a reduction in the intensity of staining of components, presenting an increase only for polysaccharides and calcium remained unaltered. In the glands of females fed for 6 days (TG) there was a reduction in the synthesis of the components, with an increase in proteins and calcium. Contrary to expected, it was verified that the alterations were more significant in the glands of females fed for 2 days than in the ones fed for 6 days, once the hosts were inoculated with antigens of the glandular tissue obtained from females fed for 6 days. In addition, it was verified that during the secretory cycle of glands from TG (2-6 days) a, c2, c5, d and f cells were the ones which presented the most histochemical alterations.
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