Journal of Bioanalysis & Biomedicine

Serum Amyloid A4 and Ceruloplasmin Evaluated Mastitic Cattle with Escherichia coli or Staphylococcus aureus including Resistant Genes

Abstract

Verginia Mohamed El-Metwally Farag, Amany Mohamed Abd-El-Moaty, Nermin Awad Ibrahim, Samar Magdy Atwa and Mohamed Abd- EL-Naeem EL-Beskawy

This study investigated the prevalence of an important Gram-negative and a Gram-positive bacterial strain, as well as the test on a few antibiotic resistant genes and sensitivity on the two bacteria. Moreover, the relations of the two microorganisms with some Acute Phase Proteins (APPs) inflammatory marker such as Serum Amyloid A (SAA) and Ceruloplasmin (Cp) in serum and milk were inspected. Out of 120 samples of Mastitic cow’s milk were identified 19.2% Escherichia coli (E. coli) and 14.2% Staphylococcus aureus (S. aureus) single isolates. Isolates of E. coli were completely sensitive to imipenem followed by meropenem then amikacin, enrofloxacin, gentamicin and cefoprazone. Two out of four isolates of multidrug resistance (against cefotaxime, penicillin, cefoprazone and gentamicin) showed positive amplification for beta lactam encoded gene blaTEM. Furthermore two isolates (out of four) resisted tetracycline were positive for TetA (A) gene. In S. aureus isolates, resistance was gradually increased from amikacin, tetracycline, and cefotaxime to oxacillin till reached 100% against penicillin. All analyzed eight isolates of penicillin resistance carried blaZ gene. Likewise, Methicillin A (mecA) gene was positive in six out of eight isolates of oxacillin resistance group. Results of SAA4 and Cp levels were differed in both pathogen groups. For E. coli group, the two levels were significantly increased in serum and only Cp elevated in milk comparing with control group. Conversely, in case of S. aureus, both parameters elevated significantly in milk and only SAA4 level was significant elevated in serum.

In conclusion, resistance to β-Lactam group, methicillin resistant group and tetracycline has reached a critical level in mastitic cattle with E. coli and S. aureus. But still the most effective and available antibiotics were enrofloxacin, amikacin and gentamicin. For diagnosis, SAA4 and Cp level has helped as a rapid tool in differentiation between both organisms.