Journal of Bioanalysis & Biomedicine

The CRISPR-Cas9 System: A New Dawn in Gene Editing

Abstract

Nurul Husna Shafie, Mohamed Saleem, Emmanuel Jairaj Moses, Siti Razila Abdul Razak and Narazah Mohd Yusoff

The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats with CRISPR-associated protein 9) system is a genome editing system that is easy to design, highly specific, efficient, robust, and well suited for highthroughput and multiplexed gene editing for a variety of cell types and organisms. In this review we describe current applications of this new system, which is growing in popularity and is increasingly being employed to selectively control gene expression on a genome-wide scale.