Journal of Oncology Translational Research

Tissue-specific MSCs demonstrate differential mitochondria transfer abilities that may affect their regenerative potential

Abstract

Swati Paliwal, Rituparna Choudhury, Anurag Agrawal and Sujata Mohanty

Mesenchymal Stem Cells (MSCs) have shown remarkable regenerative potential to treat many diseases through various mechanisms. Mitochondria transfer through stem cell has emerged as an efficient strategy to repair and regenerate damaged cells in asthma, damaged alveolar cells in lung and renal tubular cells. Though mitochondria transfer through stem cells has also been observed in case of cardiac and neuronal cells, but the ability of regeneration has not been well investigated. In this study, we have compared regenerative potential of MSCs from different tissue origins and shown their ability to repair cardiac and neuronal cells under oxidative stress. MSCs were obtained from different tissue sources such as bone marrow (BM), adipose (AD), dental pulp (DP) and Wharton’s jelly (WJ). Antimycin A has been used to induce oxidative stress by elevating levels of reactive oxygen species (ROS). Cardiac and neuronal cells under oxidative stress were stained with cell trace violet and then co-cultured with tissue-specific MSCs whose mitochondria were labeled green with Mitotracker green.