After blocking (3% BSA in TBS-T for 30 min), incubating with HRPconjugated anti-actin antibody (Abcam, ab49900), diluted in 3% BSA, TBS-T for 30 min and washing with TBS-T three times, membranes were reprobed with a mild stripping buffer composed of 0.1 M glycine, 0.2 M NaCl, and 0.1% Tween-20 at pH 2.5. The ChemiDoc XRS system was used to photograph membranes (Biorad, Hercules, CA, USA). Signals were standardised to actin and band densities were determined using NIH Image J programme. Both in vitro and in vivo investigations demonstrated antiviral activity to be a more effective method of stopping CHIKV replication. In order to overcome increased inhibition, ongoing research in our lab is concentrating on the encapsulation of several vs iRNAs.