Journal of Bioprocessing & Biotechniques

The production of ammonium and lactic acid is a major challenge in industrial animal cell culture, affecting cell growth, productivity, and product quality. Ammonium and lactic acid are by-products of metabolism, specifically from the breakdown of amino acids and glucose, respectively. Their accumulation can lead to a decrease in culture pH, cell viability, and overall productivity. Therefore, strategies to lower the production of these metabolites are crucial for optimizing cell culture processes. This article explores quick and effective methods to reduce the production of ammonium and lactic acid in industrial animal cell culture.

Anaerobic fungi are important members of the rumen microbiome, playing a crucial role in the degradation of plant biomass. However, their full potential in industrial applications, such as biofuel production, has not been fully realized. One approach to enhance the efficiency of anaerobic fungi is to create microbial consortiums with compatible partners. In this article, we discuss the use of in silico methods to identify potential microbial partners for anaerobic fungi, focusing on their metabolic interactions and the benefits of consortiums in biomass degradation.

Purine derivatives are important compounds with diverse applications in the food and pharmaceutical industries. Traditional methods for their production are often complex and inefficient. In this article, we discuss a novel approach using one-pot multi-enzymatic reactions for the efficient synthesis of purine derivatives. We explore the advantages of this approach, its applications in the food and pharmaceutical industries, and future prospects for this technology. Purine derivatives are a class of organic compounds widely recognized for their significant roles in biological processes and their extensive applications in the food and pharmaceutical industries. They are key components of nucleotides, which are fundamental to DNA and RNA, and play crucial roles in cellular metabolism. The demand for purine derivatives is growing, driving the need for efficient and sustainable production methods. One-pot multi-enzymatic synthesis represents an innovative approach to produce these compounds efficiently and sustainably. This article explores the principles, methods, and applications of one-pot multi-enzymatic production of purine derivatives, focusing on their use in the food and pharmaceutical industries.

The synthesis of retinyl laurate, a valuable nutraceutical compound, using lipase-catalyzed esterification is a promising approach. However, traditional methods suffer from low efficiency and yield. In this article, we propose a novel method that integrates artificial neural network (ANN) optimization with ultrasound support to enhance the lipase-catalyzed synthesis of retinyl laurate. We discuss the advantages of this approach, its potential applications in the food and pharmaceutical industries, and future research directions. Retinyl laurate is a compound derived from vitamin A (retinol) and lauric acid, known for its potential health benefits in skin care and nutrition. Traditional chemical synthesis of retinyl laurate is inefficient and involves the use of toxic reagents. Lipase-catalyzed esterification offers a more sustainable and efficient approach for the synthesis of retinyl laurate. However, the efficiency of this process can be further enhanced by optimizing reaction conditions. In this article, we propose a novel method that integrates ANN optimization with ultrasound support to improve the lipase-catalyzed synthesis of retinyl laurate.