Journal of Bioprocessing & Biotechniques

The accumulation of ammonium and lactic acid is a significant challenge in industrial animal cell culture, impacting cell growth, productivity and product quality. These by-products result from the breakdown of amino acids and glucose, respectively. Their buildup can lower the culture pH, diminish cell viability and reduce overall productivity. Consequently, effective strategies to minimize their production are essential for optimizing cell culture processes. This article investigates rapid and efficient techniques to reduce ammonium and lactic acid production in industrial animal cell cultures.

Anaerobic fungi are vital components of the rumen microbiome, significantly contributing to plant biomass degradation. Despite their potential, their application in industrial processes like biofuel production remains underutilized. One effective strategy to boost the efficiency of anaerobic fungi is to form microbial consortiums with compatible partners. This article explores the use of in silico methods to identify suitable microbial partners for anaerobic fungi, emphasizing their metabolic interactions and the advantages of consortiums for enhancing biomass degradation.

The synthesis of retinyl laurate, a valuable nutraceutical compound, through lipase-catalyzed esterification presents a promising approach. However, traditional methods often face challenges with low efficiency and yield. This article introduces an innovative method that combines Artificial Neural Network (ANN) optimization with ultrasound support to enhance the lipase-catalyzed synthesis of retinyl laurate. Retinyl laurate, derived from vitamin A (retinol) and lauric acid, is known for its beneficial effects in skin care and nutrition. Traditional chemical synthesis methods are not only inefficient but also involve toxic reagents. Lipase-catalyzed esterification offers a more sustainable and effective alternative. By integrating ANN optimization with ultrasound assistance, this novel approach aims to further improve the efficiency and yield of the synthesis process. We discuss the benefits of this integrated method, explore its potential applications in the food and pharmaceutical industries and suggest directions for future research to advance this technology.

Purine derivatives are crucial compounds with broad applications in the food and pharmaceutical industries. Traditional production methods for these compounds are often complex and inefficient. This article introduces a novel approach utilizing one-pot multi-enzymatic reactions for the efficient synthesis of purine derivatives. We delve into the benefits of this method, its applications in the food and pharmaceutical sectors, and future advancements in this technology. Purine derivatives, essential components of nucleotides, play vital roles in biological processes such as DNA and RNA synthesis and cellular metabolism. The growing demand for these derivatives underscores the need for more efficient and sustainable production techniques. One-pot multi-enzymatic synthesis offers a promising solution, providing a streamlined and eco-friendly approach to produce purine derivatives. In this article, we examine the principles and methodologies behind one-pot multi-enzymatic synthesis, highlighting its applications in the food and pharmaceutical industries and discussing the future potential of this innovative technology.