Journal of Forensic Research

Forensic analysis of mixed DNA samples poses a potential challenge for the investigator. Male-female mixed DNA samples in sexual assault cases are commonly analyzed in forensic laboratory but in case of male-male and female-female mixed DNA sample from multiple perpetrators in sexual assaults (gang rapes) and homicide cases, it is not easy to discriminate the origin of different donors. We present a protocol for mixed blood stain samples involving agglutination of leukocytes with ABO antisera and using the separated leukocytes for DNA profiling. By using this technique, we were able to differentiate the leukocytes of different individuals. DNA profiles were obtained using AmpFlSTR identifiler plus amplification kit.