Molecular and Genetic Medicine

Radiation therapy (RT) is an important component of modern cancer management. RT inherent more benefits as compared to both chemotherapy and surgical intervention due to its cost effectiveness, targeted effects and organ saving properties. Despite the advancement in the field of radiation delivery, radiation induced side effects; mainly to the skin is the limiting factor in delivering high intensity therapeutic doses.

A neurosurgery is made by Helicase 4 to 6 genes of Epstein Barr Virus (4 to 6 Helicase gene of EBV); for this was it is made to surge tumor cells in patients of Medulloblastoma and Glioma in both animals and human clinical trial phase-III. In this case it will act by surgical procedure himself (surgical Neurooncology procedure) by acting as neurosurgeon inside tumor cells in both patients of Medulloblastoma and Glioma of human and mice phase –III; in this case the well doing is seizure of gene 5 of EBV and the other is scalpel. Of these tumors (Medulloblastoma and Glioma) of animal cells and human 10% were fully treated by removing NOTCHES of c-AMP of Medulloblastoma (neuroprimitive ectodermal tumors of cerebellum in embryo and children) and primitive neuroectodermal sheath of skull (Glioma in young and adults) by Helicase gene 4 and 6 (scalpel process). Other issue is to find a medium inside cerebrospinal fluid (CSF) of both tumors by Helicase gene itself in destroying plasmodesmata between cells of tumor by artificial plasmosomes (synthesized in self-funded laboratories). The EBV is fully power in its enzyme (Helicase gene from 4 to 6 with fully length density 2000 of plasmosomes due to it will act as a neurosurgeon in its needed. Other thing it will act as scalpel in its issue without any toxic side effects.

Introduction: Breast cancer is one of the most common types of cancer among women and is the first cause of cancer deaths in women. According to world health statistics, one out of every 8 to 10 women develops breast cancer. According to Iran statistics, out of every 10 to 15 women in our country, one woman has probably breast cancer, so the aim of this study was to investigate the relation between ADSL gene rs3788577 polymorphism, rs7816345 polymorphism (near znf703 gene) and rs17001868 polymorphism associated with SGSM3 gene with breast cancer in the population of East Azerbaijan.

Methods: In this study, 100 samples from patients with breast cancer and 100 blood samples from healthy individuals were selected as control group. Then, according to the protocol, DNA was extracted from all samples with the DNA extraction kit. Electrophoresis was then carried out to assure the quality of the extracted DNA, and quantified by spectrophotometer. The samples were then PCR-amplified with specific primers and finally "PCR products were treated with the restriction enzyme and electrophoresed on agarose gel". Data were analyzed by SPSS software version 10 using descriptive and chi-square tests and significance level less than 0.05 was considered.

Results: The results of ADSL gene rs3788577 polymorphism analysis showed that the percentage of G allele was 14.5% and 18.1% in healthy and diseased individuals, respectively. Examination of these data shows that the G-allele has a 44% increase in diseased people compared to healthy people. The results of SGSM3 gene polymorphism 17001868 showed that the percentage of T-allele was 15% and 21.5%, respectively. The examination of these results showed that T-allele increased 6.5% in healthy individuals and also rs7816345 polymorphism results showed that the percentage of T-allele in healthy subjects was reported to be 44.5% and 69.5%. Examination of these data shows that the T-allele has an 11% increase in diseased people compared to healthy people.

Discussion: Polymorphism analysis of rs378857 rs7816345 showed that there is probably a relationship between increased G allele (44%) and increased T allele (25%) respectively and the incidence of breast cancer and its prevalence in Azerbaijan population. On the other hand, rs 17001868 polymorphism analysis showed that there is "probably no relation between the T allele increase (by 6.5%) and the incidence of breast cancer.

Conclusion: Given that current methods of treatment for all types of cancers have serious consequences, discovering new ways to diagnose the disease early by identifying specific biomarkers for that type of cancer is essential and can open new therapeutic horizons.