Journal of Molecular Histology & Medical Physiology

Objectives and method: To research the parameters of perfusion of the functioning portion of the rats′ small intestine in the chronic experiments under physiological condition without operation trauma, pain, narcosis and atrophy of the small intestine. To determine the volumes of the outflowting perfusate and the dependence of the velocity of glucose absorption in the functioning portion of the rats′ small intestine on the rate of its perfusion.
Results: At perfusion of the functioning portion of the small intestine of rats for each 5 min it is possible to collect 50-70% of the perfusion solution introduced into the researching area. The volume of outflowing perfusate is largely determined by peristalsis, by the degree of satiety of the animal and by its functional state. In the range of the changing of the rate of perfusion from 0.37 to 2.61 ml/min the velocity of absorption of glucose from its 25 mmol/l solution changes from 7.63 ± 0.38 to 10.33 ± 1.90 mcmol/min and concentration of unabsorbed marker PEG changes from 102.89 ± 0.87 to 98.28 ± 1.85% correspondingly. The minimal velocity of glucose absorption and minimal concentration of PEG were detected under the velocity of perfusion 5.39 ± 0.36 ml/min: 1.12 ± 2.28 mcmol/min and 95.07 ± 2.93% correspondingly. In the range of the rate of perfusion from 0.37 ± 0.01 to 1.43 ± 0.04 ml/min there were detected absorption of water from the perfusate. Dilution of the perfusate starts only under the rate of perfusion 2.61 ± 0.04 ml/min and increases under the 5.39 ± 0.36 ml/min.
Conclusions: The volume of outflowing from the functioning portion of the small intestine of the rats’ perfusate is largely determined by peristalsis, by the degree of satiety of the animal and its functional state. The velocity of glucose absorption from its 25 mmol/l solution in the functioning portion of the rats′ small intestine is not very sensitive to the rate of the perfusion in the range from 0.37 ± 0.01 to 2.61 ± 0.04 ml: it changes on 26% at the changing of the rate of perfusion on 86%.

A number of diagnostic and research assays rely on accurately measuring the levels of ribonucleic acids (RNAs) in tissue. The best way to obtain high quality intact RNA from tissues is to use samples that are fresh. The standard for treating tissues used in pathology workflow is to treat with 10% neutral buffered formalin, alcohols, xylenes and waxes but produces lower quality material for detection of RNA. One barrier to analyzing RNA species in formalinfixed paraffin embedded tissues (FFPE) is the use of variable times that institutions fix tissue samples which causes variable preservation and assay results. Another barrier is how long samples sit at room temperature (RT) before fixing, so called cold ischemia time. We report here on increased detection of RNA species from our rapid twotemperature formalin fixation protocol (cold+warm) which standardizes tissue collection and reduces cold ischemic time as variables in RNA ISH analysis. Human tonsils, mouse xenograft tumors and cell culture samples were used as model systems in fixation time course experiments to determine sensitivity of RNA preservation to the level of fixation. To extend these results colon, breast and lung carcinoma clinical patient samples were also examined. The amount of detectable β-actin RNA or miRNAs increased with increasing fixation times in model systems. In clinical tissues, the amount of detectable RNA species dramatically increased (20-40 fold) in tissues fixed with the cold +warm method. A dramatic reduction (20-50 fold) in RNA levels was observed when the tissues had longer cold ischemia times with standard room temperature fixation.

Tuberculosis is a very important disease worldwide. It affects many parts of the body. Concerning the testis, the author documented personally the diseases affecting the Igbos, a large Ethnic Group in Nigeria. Therefore, the present paper considers the findings as regard testicular tuberculosis. It was striking that as many as 16 doctors each submitted a case of testicular tuberculosis during a period of 30 years. It is concluded that this is like the single case report usually found in the literature of this infectious illness.