Journal of Phylogenetics & Evolutionary Biology

Microorganisms (microbes, protozoa, diseases, etc.) in water are a crucial indicator of its security and quality. In water quality control labs, these bacteria are typically located using conventional and antiquated methods; however, the speed and accuracy of the results are generally constrained by these methods. The methods for examining water have significantly changed thanks to the application of subatomic science. However, the choice of the fixation convention taking the greatest rate of microbial recovery in a suspension into consideration continues to be a real test. This preliminary review's objective is to examine the rate of recovery of three distinct water focus conventions (film filtration, filtration on cloth cushion, and centrifugation) for tests intended for research.

Plastid phylogenomics has emerged as a powerful tool for studying evolutionary relationships and resolving taxonomic uncertainties in various organisms. Plastids, being semi-autonomous organelles with their own genomes, provide a wealth of genetic information that can be harnessed to elucidate phylogenetic relationships and discriminate between closely related species. This manuscript provides a comprehensive overview of the applications and advancements in plastid phylogenomics for species discrimination. We discuss the principles of plastid genome organization, the methodologies employed in plastid phylogenomics, and the significant contributions of plastid phylogenomics towards species discrimination. We also highlight the challenges and future prospects of this rapidly evolving field.

Nerve tissue engineering has emerged as a promising field that has the potential to revolutionize the treatment of neural injuries and degenerative disorders. This abstract provides an overview of the advancements in nerve tissue engineering, focusing on the use of cells, growth factors, scaffolds, and manufacturing processes to unlock the potential for regenerating neural tissue. Cells play a crucial role in nerve tissue engineering, and researchers are exploring various cell sources, such as neural stem cells, Schwann cells, and induced pluripotent stem cells, to harness their therapeutic capabilities. Growth factors, including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), and Glial Cell Line- Derived Neurotrophic Factor (GDNF), are employed to support cell survival, promote the growth of nerve fibers, and facilitate tissue regeneration. Scaffolds are used to provide structural support and guidance for cell growth and the regrowth of nerve fibers. Biomaterials such as hydrogels, electrospun fibers, and 3D-printed scaffolds offer customizable properties that can closely mimic the natural microenvironment of nerves.

An increasing corpus of research contends that many biological processes are driven by phase separation occurring within polymer mixtures. Many different roles in cellular metabolism, gene regulation, and signalling are assumed to be played by the development of membrane-less organelles by liquid-liquid phase separation. One of these systems' traits is that it is poised at the edge of a phase transition, which makes it ideal for inducing strong cellular reactions to frequently extremely little changes in the cell's “environment.” Recent research suggests that phase separation may be a driving force for cell wall expansion in the semisolid environment of plant cell walls, as well as a factor in wall patterning, hydration, and stress relaxation during growth.