United States
Research Article
Increased Detection of RNA Species in Histological Tissues Using a Two-Temperature Fixation Protocol
Author(s): Abbey Theiss P, David J, Daniel Bauer R, Pedata A, Dipti T, Mark Robida D, Michael O, William Day A and David Chafin RAbbey Theiss P, David J, Daniel Bauer R, Pedata A, Dipti T, Mark Robida D, Michael O, William Day A and David Chafin R
A number of diagnostic and research assays rely on accurately measuring the levels of ribonucleic acids (RNAs) in tissue. The best way to obtain high quality intact RNA from tissues is to use samples that are fresh. The standard for treating tissues used in pathology workflow is to treat with 10% neutral buffered formalin, alcohols, xylenes and waxes but produces lower quality material for detection of RNA. One barrier to analyzing RNA species in formalinfixed paraffin embedded tissues (FFPE) is the use of variable times that institutions fix tissue samples which causes variable preservation and assay results. Another barrier is how long samples sit at room temperature (RT) before fixing, so called cold ischemia time. We report here on increased detection of RNA species from our rapid twotemperature formalin fixation protocol (cold+warm) which standardizes tissue collection and reduce.. Read More»
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