Journal of Bioprocessing & Biotechniques

Abhishek Dutt Tripathi

Publications

• Research Article
  Statistical Optimization of Parameters Affecting Polyhydroxybutyrate(PHB) Recovery by Dispersion Method from Alcaligenes Cells and Its Characterization
  Author(s): Abhishek Dutt Tripathi and Suresh Kumar SrivastavaAbhishek Dutt Tripathi and Suresh Kumar Srivastava
  
  In the present study, efforts have been made to optimize the three process variables i.e., incubation time, temperature of separation and hypochlorite concentration for enhanced polyhydroxybutyrate (PHB) recovery from Alcaligenes cells which may serve as precursor for bio-plastic production. Screening of bio-plastic (PHB) producing microorganism was done by TEM (Transmission electron microscopy). Initially, three recovery methods were applied to achieve efficient PHB recovery which included; Alkali treatment, ATPS (Aquas two phase separation (PEG 6000)/ KH2PO4) and chloroform-hypochlorite dispersion (1:1, v/v). Maximum PHB recovery of 95 ± 0.5% with 97.0 ± 0.29% purity was obtained by dispersion method. In order to enhance the PHB recovery further, dispersion process was optimized using design expert (DX 8.0.6) software. PHB.. Read More»
  DOI: 10.4172/2155-9821.1000238

  Abstract PDF