Veterinary Science & Technology

Absalón-Medina VA

Publications

• Research Article
  Antioxidant Effects of Seminal Plasma on Cellular Morphological Viability of Swine Semen Post-Cryopreservation
  Author(s): Hernández DAK, Barrientos-Morales M, Cervantes AP, Hernández BA, Domínguez MB and Absalón-Medina VAHernández DAK, Barrientos-Morales M, Cervantes AP, Hernández BA, Domínguez MB and Absalón-Medina VA
  
  The aims of this study were to measure the antioxidant enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX1) in seminal plasma (SP), the influence of temperature overtime on such enzymes and, to assess post-thaw viability of semen supplemented with autologous or homologous SP by nigrosine-eosin (NE) and hypoosmotic tests (HOST). A total of 48 sperm-rich fractions from 8 boars were collected and equally divided for SP antioxidant activity determination or cryopreservation experiments. Marked differences in SP antioxidant activity were found amongst individuals. SOD values ranged from 7.88 ± 0.04 U/g to 12.01 ± 0.07 U/g. Restricted maximum likelihood variance components estimates (REML) indicated that 98% of the variation resided between individuals (p<0.05). In addition, GPX1 activity ranged from 0.03 ± 0.001 U/g .. Read More»
  DOI: 10.4172/2157-7579.1000225

  Abstract PDF