Sudan
Research Article
A Comparison Study of Histochemical Staining of Various Tissues after Carnoy's Versus after Formalin Fixation
Author(s): Hussain Gadelkarim Ahmed and Ahmed Ibn idris MohammedHussain Gadelkarim Ahmed and Ahmed Ibn idris Mohammed
Background: The potential problems related to the use of formalin in histopathology, such as health hazards, deterioration of nucleic acids are well-known. The aim of this study was to evaluate the utilization of a Carnoy's solution fixation in comparison with formalin on subsequent tissue sectioning and histochemical staining. Materials and Methods: Corresponding sections of 25 tissue biopsies of rabbit's different organs were fixed in Carnoy's solution and in 10% neutral buffered formalin. Samples were processed using the conventional method and then stained applying five histochemical methods. The degree of the quality of the staining was assessed for each method by scoring system (1-10) depending on comparison of the stained tissue sections with illustrated photomicrographs. Results: For the quality of cutting, the best quality was obtained by Formalin (mean = 4.76) then Carno.. Read More»
DOI:
10.4172/1948-5956.1000065
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