Journal of Diabetic Complications & Medicine

Alejandro Hernández-Seara

Publications

• Research Article
  Epidermal Growth Factor based Therapy Promotes Intracellular Trafficking and Accumulation of its Receptor in the Nucleus of Fibroblasts from Diabetic Foot Ulcers
  Author(s): Viviana Falcón-Cama, Maday Fernández-Mayola, Yssel Mendoza-Marí, Nelson Acosta-Rivero, Ariana García-Ojalvo, Ricardo Bringas-Pérez, Ivón Menéndez-Valdés, Mariuska Matos-Terrero, Lilianne López-Noudo, Rocío Garateix-Suárez, Karla Pereira-Yañez, Maritza González, Sirenia González-Pozos, Juan Kourí-Flores, William Savigne-Gutiérrez, Ivonne Salgado, Alejandro Hernández-Seara, David G Armstrong and Jorge Berlanga-AcostaViviana Falcón-Cama, Maday Fernández-Mayola, Yssel Mendoza-Marí, Nelson Acosta-Rivero, Ariana García-Ojalvo, Ricardo Bringas-Pérez, Ivón Menéndez-Valdés, Mariuska Matos-Terrero, Lilianne López-Noudo, Rocío Garateix-Suárez, Karla Pereira-Yañez, Maritza González, Sirenia González-Pozos, Juan Kourí-Flores, William Savigne-Gutiérrez, Ivonne Salgado, Alejandro Hernández-Seara, David G Armstrong and Jorge Berlanga-Acosta
  
  Objective: To gain a better understanding of the Epidermal Growth Factor (EGF) Receptor (EGFR) activation, trafficking and biological response in diabetic foot ulcers (DFU), exposed to recombinant human EGF via intra-ulcer infiltration as a healing alternative. Methods: We studied by immunoelectron microscopy the intracellular localization of the EGFR and Proliferating Cell Nuclear Antigen (PCNA) in fibroblast-like cells (FLC) from granulation tissue of DFU patients, collected before and at different time points after EGF treatment. Results: EGF therapy appears to increase EGFR immunolabeling. At early time-points, EGFR labeling is observed predominantly in the nucleus, suggesting a fast EGFR internalization and nuclear translocation. Interestingly EGFR is also detected in the mito.. Read More»
  DOI: 10.4172/2475-3211.1000111

  Abstract PDF