Journal of Tissue Science and Engineering

Alex Odermatt

Publications

• Research Article
  Characterization of a Rat Multi-Cell Type 3D-Liver Microtissue System
  Author(s): Denise V Kratschmar, Simon Messner, Wolfgang Moritz and Alex OdermattDenise V Kratschmar, Simon Messner, Wolfgang Moritz and Alex Odermatt
  
  Abstract Background: Primary hepatocytes rapidly lose their polarized morphology and the expression of important liverspecific metabolic enzymes, receptors and transport proteins under normal two-dimensional (2D) culture conditions. Thus, their use as a reliable predictive in vitro model for drug-induced liver injury is limited. Three-dimensional (3D) liver micro tissue culture systems have become an increasingly attractive alternative for the evaluation of druginduced liver toxicity. However, several liver-specific pathways remain to be characterized in such models. Methods and principal findings: In the present work, we compared the expression of several genes with a role in the anti-oxidant cell defense and glucocorticoid pathways in rat H4IIE hepatoma cells, primary rat hepatocytes, 2D-hepatocyte sandwich culture, and a multi-cell type micro tissue model comprisin.. Read More»
  DOI: 10.4172/2157-7552.1000130

  Abstract PDF