Journal of Bioanalysis & Biomedicine

Amal Shervington

Publications

• Research Article
  Validating the Efficacy of Shrna Vs Sirna In Silencing Hsp90�Ž�± In Gliomas
  Author(s): Chinmay Munje, Leroy Shervington, Adi Mehta, Zarine Khan and Amal ShervingtonChinmay Munje, Leroy Shervington, Adi Mehta, Zarine Khan and Amal Shervington
  
  The stress dependent tumour cells found to harbour Hsp90 and its inducible component Hsp90á in activated superchaperone complexes are highly sensitive to pharmacological Hsp90 inhibitors compared to normal cells where Hsp90 is present in an uncomplexed state. Downregulating Hsp90á can be achieved using chemical inhibitors and RNAi such as siRNA’s or shRNA’s. This study aimed at assessing the efficacy of siRNA (targeting hsp90á exon 5) and shRNA (targeting hsp90á exon 4 and 5 border) in three glioma cell lines (1321N1, GOS-3 and U87-MG). hsp90á expression at mRNA and protein levels was monitored using qRT-PCR and immunofluorescence, respectively. The downstream effect of silencing hsp90á was determined by measuring the Akt/PKB kinase activity level. While siRNA treatment decreased hsp90α mRNA copy numbers by ~35%, shRNA decreased it b.. Read More»
  DOI: 10.4172/1948-593X.S3-002

  Abstract PDF