Veterinary Science & Technology

Ana Carolina Carvalho

Publications

• Research Article
  Detection of Animal Products in Ruminant Feeds by Microscopy and Real Time PCR
  Author(s): Luciana Pacheco Golinelli, Joab Trajano Silva, Ana Carolina Carvalho and Vânia Margaret Flosi PaschoalinLuciana Pacheco Golinelli, Joab Trajano Silva, Ana Carolina Carvalho and Vânia Margaret Flosi Paschoalin
  
  This study evaluated the performance of the TaqManTM real- time PCR assay to detection of DNA from not allowed-animal derivatives in reference feeds samples. The results of qPCR were compared with the microscopy, only method validated to control the presence of animal proteins, according the European Communities. The qPCR tests targeting 12S rRNA from cows, sheep, porcine and chickens and cytochrome b region from caprine in feeds were able to detect half the amount (0.0125% w/w) of meat-and-bone meal (MBM) that could be detected by microscopy in samples spiked with MBM. Although cross-contamination in feeds and food processing plants is an unexceptional problem, the presence of traces of prohibited animal products in feedstuffs is an alert to potential impact on herd and human health, because it has been associated with the transmission of transmissible spongiform encephalopathy (TSE).. Read More»
  DOI: 10.4172/2157-7579.1000316

  Abstract PDF