Veterinary Science & Technology

Annabell C Segarra

Publications

• Research Article
  Comparison of Two Methods of Estradiol Replacement: their Physiological and Behavioral Outcomes
  Author(s): Laurivette Mosquera, Luz Shepherd, Aranza I Torrado, Yvonne M Torres-Diaz, Jorge D Miranda and Annabell C SegarraLaurivette Mosquera, Luz Shepherd, Aranza I Torrado, Yvonne M Torres-Diaz, Jorge D Miranda and Annabell C Segarra
  
  Fluctuating sex steroids during the estrous or menstrual cycle of mammalian females make it difficult to determine their role on behaviors and physiology. To avoid this, many investigators ovariectomize their animals and administer progesterone, estradiol or a combination of both. Several different strategies are used to administer estradiol, which confounds interpretation of results. This study compared two methods of estradiol replacement implants: Silastic tubes filled with crystalline estradiol benzoate (E2) and commercially available estradiol benzoate pellets. Implants were placed subcutaneously in adult ovariectomized (OVX) rats and blood samples obtained weekly. Control OVX rats received empty Silastic tubes or placebo pellets. Our data shows that E2 plasma levels from rats with Silastic implants peaked after one week and decreased slowly thereafter. In contrast, plasma E2 fro.. Read More»
  DOI: 10.4172/2157-7579.1000276

  Abstract PDF