Nuclear Medicine & Radiation Therapy

Anne-Laure Boulesteix

Publications

• Research Article
  The Radiation Induced Migration of Human Malignant Glioma Cells can be Blocked by Inhibition of the EGFR Downstream Pathways
  Author(s): Anja Pickhard, Johanna Margraf, Guido Piontek, Andreas Knopf, Rudolf Reiter, Anne-Laure Boulesteix and Jürgen SchlegelAnja Pickhard, Johanna Margraf, Guido Piontek, Andreas Knopf, Rudolf Reiter, Anne-Laure Boulesteix and Jürgen Schlegel
  
  Background: It is well documented that low dose ionizing radiation induces migration of glioma cells, but the mechanisms are still poorly understood. The aim of the current study was to elucidate the intracellular signal transduction pathways of radiation induced migration in human glioma cells. Methods: Migration was assessed via a wound healing assay. In addition, tumor cell proliferation was evaluated with a MTT colorimeritric assay using 3 glioma cell lines (LN18, LN229, LNZ308). The cells were treated with increasing doses of irradiation (2Gy, 5Gy, 8Gy) in the presence or absence of EGF or inhibitors of the EGFR or downstream pathways (AG1478, LY294002, PD98059). Biochemical activation of EGFR, Akt/PKB and MAPK/ERK was examined by Western blot analysis. Results: Irradiation induced a dose dependant intense increase of migrating cells and a dec.. Read More»
  DOI: 10.4172/2155-9619.1000138

  Abstract PDF