Journal of Computer Science & Systems Biology

Antonio Gómez

Publications

• Research Article
  OMICS Techniques and Identification of Pathogen Virulence Genes Application to the Analysis of Respiratory Pathogens
  Author(s): Sergio Hernández, Antonio Gómez, Juan Cedano and Enrique QuerolSergio Hernández, Antonio Gómez, Juan Cedano and Enrique Querol
  
  The advent of genomics should have facilitated the identification of microbial virulence factors, a key objective for vaccine design, especially for live attenuated vaccines. It is generally assumed than when the bacterial pathogen infects the host it expresses a set of genes, a number of them being virulence factors. However, up to now, although several Omics methods have been applied to identify virulence genes, i.e., DNA microarrays, In Vivo Expression Technology (IVET), Signature-Tagged Mutagenesis (STM), Differential Fluorescence Induction (DFI), etc., the results are quite meager. Among the genes identified by these techniques there are many related to cellular stress, basal metabolism, etc., which cannot be directly involved in virulence, or at least cannot be considered useful candidates to be deleted for designing a vaccine. Among the genes d.. Read More»
  DOI: 10.4172/jcsb.1000024

  Abstract PDF