Veterinary Science & Technology

Arnaud Van Wettere J

Publications

• Research Article
  Estimation of the Trichomonad Concentration in Beef Bull Preputial Samples and Resultant Population Effect of Pooling on Detection of Tritrichomonas foetus Using qPCR
  Author(s): David J. Wilson, Thomas J. Baldwin, Amanda Wilhelm, Chelsea H. Whitehouse, Jennifer L. Bunnell, Kerry A. Rood and Arnaud Van Wettere JDavid J. Wilson, Thomas J. Baldwin, Amanda Wilhelm, Chelsea H. Whitehouse, Jennifer L. Bunnell, Kerry A. Rood and Arnaud Van Wettere J
  
  The primary objective was assessment of effect of sample pooling on Tritrichomonas foetus detection using qPCR in beef bulls within 5 logs of trichomonad concentration in spiked preputial samples. The secondary objective was to use qPCR Ct values to estimate the percentage of beef bulls in the field population within each log of trichomonad concentration. Solutions containing none, 1,10,100,1000 and 10,000 trichomonads (T)/ml were made and tested by qPCR. Test sensitivities for single positive samples were: 1 T/ml, 20/40 (50%); 10 T/ml, 40/40 (100%); 100 T/ml, 40/40 (100%); 1000 T/ml, 40/40 (100%); 10,000 T/ml, 40/40 (100%). For 5-sample pools (1 positive and 4 negative), sensitivities were: 1 T/ml, 6/40 (15%), 10 T/ml, 26/40 (65%); 100 T/ml 40/40 (100%); 1000 T/ml, 40/40 (100%); 10,000 T/ml, 40/40 (100%). Specificity for both single and pooled negative samples was 40/40 negative (100.. Read More»
  DOI: 10.4172/2157-7579.1000434

  Abstract PDF